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I found the paradigm used by the authors to be appropriate for examining the extent to which humans are able to identify whether sequentially heard screams were produced by the same vocaliser. The ecological validity of this paradigm to the 'real world' problem of being able to identify which of 30 odd group-members produced an unseen scream (as demonstrated in primate literature) is debatable, but this is acknowledged by the authors in their Discussion. However, it would greatly improve the Introduction if these limitations were also outlined there, as well as how the specific aims of this study will inform the broader question. Currently, this link is not as clear as it could be.The methods were very clearly and thoroughly described such that replication would be straightforward. However, the statistical software used for analysis is not specified, which I would encourage. If R was used, then the relevant scripts would be a welcome addition to the supplemental materials.My biggest methodological concern is that the authors approach to acoustic analysis was somewhat unconventional. They have taken a qualitative (two researchers deciding which screams are similar), rather than quantitative (directly measuring acoustic parameters to determine which are similar), approach towards determining similarity between screams. I think the manuscript would greatly benefit from a justification and cited precedent for the methods used here, or else a more formal acoustic analysis.

The system described has the potential to be used for engineering the functional responses of mature guard cells as it was observed that expression of the reporter genes are spatially restricted only to stomatal guard cells. In addition, the system that has been developed here is highly sensitive to the inducer (ethanol), as concentrations as low as 4% ethanol supplied as a vapour is sufficient to induce expression of the reporter genes. The ability for the spatio-temporal control of expression of multiple genes in stomatal guard cells will be an important tool for dissecting the complex signalling system utilized by stomatal guard cells to sense and respond to changes in the prevailing environmental conditions.

In a series of experiments between 1960 and 1965, Robert Geoffrey Edwards discovered how to make mammalian egg cells, or oocytes, mature outside of a female's body. Edwards, working at several research institutions in the UK during this period, studied in vitro fertilization (IVF) methods. He measured the conditions and timings for in vitro (out of the body) maturation of oocytes from diverse mammals including mice, rats, hamsters, pigs, cows, sheep, and rhesus monkeys, as well as humans.

Somites are blocks of mesoderm that are located on either side of the neural tube in the developing vertebrate embryo. Somites are precursor populations of cells that give rise to important structures associated with the vertebrate body plan and will eventually differentiate into dermis, skeletal muscle, cartilage, tendons, and vertebrae. Somites also determine the migratory paths of neural crest cells and of the axons of spinal nerves.

Ovarian hyperstimulation syndrome, abbreviated OHSS, is an atypical reaction that women may experience in response to excessive hormones, and often occurs during fertility treatments. OHSS is typically triggered by hormonal medications designed to mature eggs in the ovaries, which can cause blood vessels within the ovaries to leak fluid. Sometimes that can lead to painful tenderness or swelling. In severe cases of OHSS, that fluid can leak into the abdominal cavity in large amounts, causing vomiting, blood clots, and severe pain. 041b061a72


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